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KMID : 0380620150470010126
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Korean Journal of Food Science and Technology
2015 Volume.47 No. 1 p.126 ~ p.131
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Prevalence and Classification of Escherichia coli Isolated from bibimbap in Korea
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Lee Da-Yeon
Lee Joo-Young
Wang Hae-Jin
Shin Dong-Bin
Cho Yong-Sun
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Abstract
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Pathogenic Escherichia coli is recognized as an important cause of diarrhea, hemorrhagic colitis and hemolyticuremic syndrome worldwide. This study was conducted to investigate the prevalence E. coli contamination in the Korean traditional food bibimbap. E. coli were isolated from 84 of 1142 (7.3%) bibimbap investigated from 2005 to 2011. Antibiotic resistance profiling demonstrated that 6 of the 84 isolates (7.2%) showed multiple drug resistance. Fifteen virulence genes specific for pathogenic E. coli such as Shiga toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and enteroaggregative E. coli (EAEC) were examined by multiplex PCR for mixed bacterial cultures derived from bibimbap samples. The EPEC virulence gene (ent) was detected in 5 strains (5.9%), while ETEC, EAEC, and EIEC were not detected. STEC serotypes O103 (1.2%), O91 (1.2%), and O128 (6.0%) were found, but other serogroups such as O26, O157, O145, O111 and O121 were not detecded. Automated Repetitive-Sequence-Based PCR analysis showed different patterns.
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KEYWORD
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bibimbap, Escherichia coli, antibiotic resistance, virulence gene, genetic diversity
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